Abstract：A bacteria consortium ( LV-1) , which can utilize di-n-butyl phthalate ( DBP) as sole source of carbon and energy, was isolated from activated sludge collected from a garbage incineration power plant of Kaifeng. Based on the results of Illumina sequencing, LV-1 contains 33 families and 48 genera of bacteria, the dominant genera is Brucella sp. and Sinobacter sp. and their relative abundance is 62. 78% and 14. 83%, respectively. The optimal degradation condition of LV-1 to degrade DBP was: temperature 30 degrees centigrade and p H 6.0. Under the optimum conditions, LV-1 degraded about93% of 500 mg·L-1DBP within 48 h, and more than 95% of 1000 mg·L-1DBP was degraded within 72 h. The investigation of degradation kinetics at different DBP initial concentrations ( 100 ~ 500 mg·L-1) showed that DBP degradation fitted the Monod first-order kinetic equation, and the half-life ranged from 13. 64 to 19. 20 h. Gas chromatography-mass spectrometry ( GC-MS) was used to analysis the intermediates during the DBP degradation process, and the degradation pathway was proposed: DBP—→BEP—→MBP—→MEP—→PA—→—→CO2+ H2O.Besides, LV-1 can effectively utilize DMP, DEP and PA.
Keyword：Di-n-butyl phthalate; bacteria enrichment; biodegradation; metabolic pathway;
Source： Acta Scientiae Circumstantiae 2017 Vol.37 (7)
